The project will construct a proof of concept test system for brain tumour tissue models using co-cultured fluorescent -marked tumour- and normal cells.
The perfusion cell is based on a microfluidic circuit allowing diagnosis with in-situ fluorescence confocal microscopy) of neural cells. This will allow nutrient and pharmaceutical preparations to be introduced during irradiation.
Use of multi-cell type brain tissue cultures with low-energy (MeV) ion beam irradiation is an completely new and low-cost way for realistic studies of the neural cellular-level activity representative of the site of tumour eradication deep inside the patients brain.
This is inaccessible to both in-vivo studies and singlecell irradiation (where intercellular cell communication is limited).